The GC–MS analysis was carried out with an Agilent 6890N–5973N GC–MS system (Santa Clara, CA, USA) on a sample of M. aquatica EO prepared by dilution to 1:100 with n-hexane. The instrument was operating in the EI mode at 70 eV and using a HP-5MS (5% phenylmethylpolysiloxane, 30 m, 0.25 mm i.d., film thickness 0.1 µm) (J&W Scientific, Folsom, CA, USA) capillary column.
The chromatographic parameters and chromatogram analysis were the same as those reported by Nkuimi Wandjou et al. [27 (link)]. Briefly, the analytical standards of α-pinene, sabinene, β-pinene, myrcene, α-terpinene, p-cymene, limonene, 1,8-cineole, (Z)-β-ocimene, (E)-β-ocimene, γ-terpinene, terpinolene, terpinene-4-ol, α-terpineol, (E)-caryophyllene, and α-humulene were purchased from Merck (Milan, Italy) and used for peak assignments based on retention time and mass spectrum (MS). Moreover, the combination of the calculated linear retention index (RI) and MS was used to confirm the identity of the other compounds. Semi-quantitative values (peak area percentages) were obtained by peak normalization without using correction factors.
The chromatographic parameters and chromatogram analysis were the same as those reported by Nkuimi Wandjou et al. [27 (link)]. Briefly, the analytical standards of α-pinene, sabinene, β-pinene, myrcene, α-terpinene, p-cymene, limonene, 1,8-cineole, (Z)-β-ocimene, (E)-β-ocimene, γ-terpinene, terpinolene, terpinene-4-ol, α-terpineol, (E)-caryophyllene, and α-humulene were purchased from Merck (Milan, Italy) and used for peak assignments based on retention time and mass spectrum (MS). Moreover, the combination of the calculated linear retention index (RI) and MS was used to confirm the identity of the other compounds. Semi-quantitative values (peak area percentages) were obtained by peak normalization without using correction factors.
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