CK is a biomarker of muscle damage and usually increases after strenuous exercise 30 . In our study, CK levels were determined with an autoanalyzer (Hitachi 7060, Tokyo, Japan) from blood samples obtained before and 3, 24, and 48 hours after the exhaustive exercise regimen.
Exercise training would generate oxidative stress and regulate the endogenous antioxidant defense system (including GPx) 31 . The analysis of plasma collected pre-exercise and 3 and 48 h post exercise was performed with the GPx-Assay-Kit (Cayman Chemical Company, Ann Arbor, Michigan, USA).
IL-6 is a multifunctional cytokine involved in pro-inflammatory and anti-inflammatory processes 32 (link). The IL-6 response caused by exercise depends on the intensity and duration of the exercise. The analysis of serum collected pre-exercise and 3 and 48 h post exercise was performed with the IL-6 Quantikine HS ELISA Kit (R&D Systems, Inc. Minneapolis, MN, USA).
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