Antimicrobial Resistance Profiling of CC464 Isolates

We determined antimicrobial susceptibility for 21 representative CC464 isolates with genotypic polymorphisms across the range of detected resistance markers. We used Mueller-Hinton agar with 5% defibrinated horse blood and 20 mg/L β-nicotinamide adenine dinucleotide for antimicrobial susceptibility testing using the disk diffusion method for ciprofloxacin (5 μg/disk), gentamicin (10 μg/disk), tetracycline (5 μg/disk), kanamycin (5 μg/disk), amikacin (30 μg/disk), tobramycin (10 μg/diskc), and streptomycin (30 μg/disk) (29 (link),30 ). We determined MIC of ampicillin (AMP) by Etest (Oxoid, http://www.oxoid.com) and interpreted the MIC_AMP and disk susceptibility results using guidelines from the European Committee on Antimicrobial Susceptibility Testing and Clinical and Laboratory Standards Institute and interpreted them as described previously (29 (link)). We used the antimicrobial drug–susceptible isolate ARI3025 as a control. We subcultured the ST5136 isolate ARI4158 on antibiotic-free blood agar plate (ARI4158_20) 22 times to assess the stability of its antimicrobial resistance genes.

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Lopes B.S., Strachan N.J., Ramjee M., Thomson A., MacRae M., Shaw S, & Forbes K.J. (2019). Nationwide Stepwise Emergence and Evolution of Multidrug-Resistant Campylobacter jejuni Sequence Type 5136, United Kingdom. Emerging Infectious Diseases, 25(7), 1320-1329.

Publication 2019

Etest

Agar Amikacin Ampicillin Antibiotic Antimicrobial Blood Ciprofloxacin Clinical laboratory Diffusion Etest European Genes Genotypic Gentamicin Horse Kanamycin Nicotinamide adenine dinucleotide Polymorphisms Streptomycin Susceptibility Tetracycline Tobramycin

Corresponding Organization :

Other organizations : University of Aberdeen

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Variable analysis

independent variables

Antimicrobial agents tested: ciprofloxacin (5 μg/disk), gentamicin (10 μg/disk), tetracycline (5 μg/disk), kanamycin (5 μg/disk), amikacin (30 μg/disk), tobramycin (10 μg/disk), and streptomycin (30 μg/disk)
Ampicillin (AMP) minimum inhibitory concentration (MIC) determined by Etest

dependent variables

Antimicrobial susceptibility of 21 representative CC464 isolates with genotypic polymorphisms across the range of detected resistance markers

control variables

Mueller-Hinton agar with 5% defibrinated horse blood and 20 mg/L β-nicotinamide adenine dinucleotide for antimicrobial susceptibility testing
Disk diffusion method for antimicrobial susceptibility testing
Guidelines from the European Committee on Antimicrobial Susceptibility Testing and Clinical and Laboratory Standards Institute used to interpret MIC_AMP and disk susceptibility results

controls

Positive control: Antimicrobial drug-susceptible isolate ARI3025
Stability control: Subcultured the ST5136 isolate ARI4158 on antibiotic-free blood agar plate (ARI4158_20) 22 times to assess the stability of its antimicrobial resistance genes

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