ChIP was performed with the Pierce Magnetic ChIP Kit (Thermo Fisher Scientific) following the manufacturer instructions. A total of 3 µg of p53 antibody (Cell Signaling Technology; #2524) were used per IP.
Total cellular RNA was extracted using the RNeasy Kit (Qiagen, Hilden, Germany) following the manufacturer’s protocol. One-step reverse transcription and real-time PCR were performed with a Quantstudio 5 (Thermo Fisher Scientific) using QuantiTect SYBR Green PCR Kit (Qiagen) as described previously [35 (link)].
Primer sequences are listed in Supplementary TableS2 .
Total cellular RNA was extracted using the RNeasy Kit (Qiagen, Hilden, Germany) following the manufacturer’s protocol. One-step reverse transcription and real-time PCR were performed with a Quantstudio 5 (Thermo Fisher Scientific) using QuantiTect SYBR Green PCR Kit (Qiagen) as described previously [35 (link)].
Primer sequences are listed in Supplementary Table
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