MG132-treated transfected cells were collected and treated overnight with Protein-A/G. MagBeads (Yeasen) and anti-ELAVL1 (Cell Signaling Technology) or anti-β-TrCP (Cell Signaling Technology) antibodies at 4 °C. The immune complexes were centrifuged and washed, and the proteins were assessed by western blotting with anti-ELAVL1 or anti-βTrCP antibodies [24 (link)].
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