Measuring Urinary Sodium and Creatinine

Ureter urine and plasma samples were used to measure sodium and creatinine. Sodium concentrations were measured using atomic absorption spectrometer (AAnalyst 400, Perkin Elmer, Waltham, MA) as described before [15 (link)]. Creatinine levels were determined using a commercially available assay kit (catalog number K625-100; BioVision, Mountain View, CA) as published [25 (link)]. GFR, as a function of creatinine clearance, was determined using the following equation: urinary volume × urinary creatinine ÷ plasma creatinine.

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Pokkunuri I., Ali Q, & Asghar M. (2016). Grape Powder Improves Age-Related Decline in Mitochondrial and Kidney Functions in Fischer 344 Rats. Oxidative Medicine and Cellular Longevity, 2016, 6135319.

Publication 2016

AAnalyst 400 K625-100

Assay Creatinine Plasma Sodium Ureter Urinary

Corresponding Organization : University of Houston

Other organizations : University of Oklahoma Health Sciences Center

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Variable analysis

independent variables

Sodium concentration measurement method (atomic absorption spectrometer)

dependent variables

Sodium concentration
Creatinine concentration
GFR (as a function of creatinine clearance)

control variables

Ureter urine and plasma samples

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