Integrin-Binding Protein Interaction Study
Corresponding Organization :
Other organizations : Shanghai University, Cleveland Clinic Lerner College of Medicine, Versiti Blood Center of Wisconsin
Variable analysis
- Flag antibody (SG4110–16, Shanghai Genomics)
- 6 × his antibody (SG4110–06, Shanghai Genomics)
- Sharpin antibody (ab174545 and ab69507, Abcam)
- FAK antibody (#3283, CST)
- Y-FAK antibodies (#3285, CST)
- PAC1 antibody (340535, BD Biosciences)
- 9EG7 antibody (553715, BD Biosciences)
- 7E2 antibody (DSHB)
- GST-fibronectin type III repeats 9–11 (GST-Fn-III)
- CDNA of full length sharpin
- CT of integrin α5β1 and integrin αIIbβ3
- Kindlin-1
- Immunoblotting
- FACS analysis
- IPTG concentration (0.4 mM) for protein expression
- Purification methods (Glutathione Sepharpose and Ni-NTA agarose)
Annotations
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