Preparation of DOPA-Capped Nanoparticles

DOPA-capped NCP nanoparticles were prepared according to our previous report16 (link). NCP@pyrolipid was prepared by adding a THF solution (80 μl) of 1,2-distearoyl-sn-glycero-3-phosphocholine, cholesterol, pyrolipid, 1,2-distearoyl-sn-glycero-3-phosphoethanolamine polyethylene glycol 2000 (2:1:0.8:1 in molar ratio) and DOPA-coated NCP to 500 μl of 30% (v/v) ethanol/water at 60 °C. The mixture was stirred at 1,700 r.p.m. for 1 min. THF and ethanol were completely evaporated and the NCP@pyrolipid solution was allowed to cool to room temperature (r.t.). NCP@pyrolipid was centrifuged at 19,650 g. for 30 min. The supernatant was then removed and the particles resuspended in PBS. ICP-MS (Agilent 7700X, Agilent Technologies, USA) was used to analyse the Pt concentration of NCP to calculate oxaliplatin loading. The particle size and zeta potential of NCP@pyrolipid in PBS were determined by Zetasizer (Nano ZS, Malvern, UK). Transmission electron microscopy (Tecnai Spirit, FEI, USA) was used to observe the morphology of NCP@pyrolipid.