Cells were seeded into 96‐well plates (1500 cells/well), and at each time point, cells were incubated with Cell Counting Kit‐8 (CCK8; Dojindo, Kumamoto, Japan) for 1.5 h, then the absorbance was measured using Microplate Manager v6.0 (Bio‐Rad). To evaluate the inhibitory effect of JPH203, 24 h after seeding, the cells were treated with the inhibitors at the indicated concentrations or the same amount of dimethyl sulfoxide (DMSO; 0.5%; vehicle control). The half‐maximal inhibitory concentration (IC50) definition and measurements were as previously reported.
8 (link)
To examine the effects of JPH203 in combination with a cyclin‐dependent kinase (CDK) inhibitor (dinaciclib, flavopiridol, or milciclib), combination assays were performed using a fixed concentration of JPH203 and three concentrations of each CDK inhibitor based on previous literature.
15 (link) Of the three concentrations tested, only the one that had the largest effect in combination with JPH203 is shown in the results.
8 (link)
To examine the effects of JPH203 in combination with a cyclin‐dependent kinase (CDK) inhibitor (dinaciclib, flavopiridol, or milciclib), combination assays were performed using a fixed concentration of JPH203 and three concentrations of each CDK inhibitor based on previous literature.
15 (link) Of the three concentrations tested, only the one that had the largest effect in combination with JPH203 is shown in the results.
