Mouse Strain Characterization of AAV

All procedures were performed as approved by the Broad Institute IACUC (0213-06-18) or Massachusetts General Hospital IACUC (2014N000005; AAVR experiments). AKR/J (000648), BALB/cJ (000651), CBA/J (000656), CAST/EiJ (000928), C57Bl/6J (000664), C57BL/J (000668), DBA/2J (000671), FVB/NJ (001800), LP/J (000676), MOLF/EiJ (000550), NOD/ShiLtJ (001976), NZB/B1NJ (000684), and PWK/PhJ (003715) were obtained from the Jackson Laboratory (JAX). AAVR KO mice were a generous gift from Dr. J.E. Carette (Stanford) to Dr. Balazs and have been previously described [39 (link)]. Recombinant AAV vectors were administered intravenously via the retro-orbital sinus in young adult male or female mice. Mice were randomly assigned to groups based on predetermined sample sizes. No mice were excluded from the analyses. Experimenters were not blinded to sample groups.

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Huang Q., Chan K.Y., Tobey I.G., Chan Y.A., Poterba T., Boutros C.L., Balazs A.B., Daneman R., Bloom J.M., Seed C, & Deverman B.E. (2019). Delivering genes across the blood-brain barrier: LY6A, a novel cellular receptor for AAV-PHP.B capsids. PLoS ONE, 14(11), e0225206.

Publication 2019

AKR/J BALB/cJ CBA/J CAST/EiJ C57Bl/6J DBA/2J FVB/NJ MOLF/EiJ NOD/ShiLtJ NZB/B1NJ PWK/PhJ

Cast Female Iacuc Male Mice Sinus Vectors Young adult

Corresponding Organization : Broad Institute

Other organizations : Ragon Institute of MGH, MIT and Harvard, University of California, San Diego

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Variable analysis

independent variables

Mouse strain (AKR/J, BALB/cJ, CBA/J, CAST/EiJ, C57Bl/6J, C57BL/J, DBA/2J, FVB/NJ, LP/J, MOLF/EiJ, NOD/ShiLtJ, NZB/B1NJ, PWK/PhJ)
Presence or absence of AAVR knockout

dependent variables

Outcomes of the experiments (not explicitly mentioned)

control variables

Intravenous administration of recombinant AAV vectors via the retro-orbital sinus
Age (young adult) and sex (male or female) of mice

positive controls

AAVR KO mice (a generous gift from Dr. J.E. Carette (Stanford) to Dr. Balazs)

negative controls

Not explicitly mentioned

Annotations

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