Quantifying Key Protein Markers in Cellular Signaling

Levels of Rab27a, IL-11, activated T-cell nuclear factor 1 (NFATc1), TRAP and GAPDH protein were analyzed using western blotting analysis, as previously described (17 (link)). The primary antibodies used were Rab27a (ab55667, 1:500), IL-11 (ab187167, 1:1,000), NFATc1 (ab25916, 1:1,000), TRAP (ab65854, 1:1,500) and GAPDH (ab8245, 1:3,000), which were all purchased from Abcam.

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Feng Q., Wang D., Feng J., Guo P, & Geng C. (2020). Denosumab inhibits MCF-7 cell line-induced spontaneous osteoclastogenesis via the RANKL/MALAT1/miR-124 axis. Translational Cancer Research, 9(4), 2482-2491.

Publication 2020

ab55667 ab187167 ab25916 ab65854 ab8245

Antibodies Gapdh Il 11 Protein T cell factor 1 Western blotting analysis

Corresponding Organization : Fourth Hospital of Hebei Medical University

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Variable analysis

independent variables

Levels of Rab27a
Levels of IL-11
Levels of activated T-cell nuclear factor 1 (NFATc1)
Levels of TRAP

dependent variables

Protein levels as analyzed using western blotting analysis

control variables

GAPDH protein levels

controls

No positive or negative controls were explicitly mentioned.

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