Immunohistochemical Analysis of KDM5B Expression

91 paired, paraffin-embedded primary specimens were stained for KDM5B by immunohistochemistry (IHC), as described previously [65 (link)]. The sections were incubated with anti-KDM5B (dilution ratio, 1:1000; No. ab181089 Abcam, Cambridge, UK) at 4°C overnight followed by secondary anti-rabbit HRP-conjugated antibody. The expression changes of KDM5B were evaluated blindly by two professional pathologists. Cancer cells were counted under the microscope as previously described [65 (link)].

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Wang Y., Liu S., Wang Y., Li B., Liang J., Chen Y., Tang B., Yu S, & Wang H. (2024). KDM5B promotes SMAD4 loss-driven drug resistance through activating DLG1/YAP to induce lipid accumulation in pancreatic ductal adenocarcinoma. Cell Death Discovery, 10, 252.

Publication 2024

anti-KDM5B ab181089

Corresponding Organization :

Other organizations : First Affiliated Hospital of GuangXi Medical University, Guangxi Medical University, Second Xiangya Hospital of Central South University, Central South University

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Variable analysis

independent variables

Staining of primary specimens for KDM5B by immunohistochemistry (IHC)

dependent variables

Expression changes of KDM5B

control variables

Paraffin-embedded primary specimens
Incubation with anti-KDM5B antibody (dilution ratio, 1:1000; No. ab181089 Abcam, Cambridge, UK) at 4°C overnight
Incubation with secondary anti-rabbit HRP-conjugated antibody
Evaluation of KDM5B expression changes by two professional pathologists in a blinded manner
Counting of cancer cells under the microscope as previously described [65 (link)]

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