Radiosynthesis and Evaluation of [11C]MPC-6827

All commercial chemicals and solvents used in the synthesis were purchased from Sigma–Aldrich Chemical Co., (St. Louis, MO), or Fisher Scientific Inc., (Springfield, NJ) and were used without further purification. Gamma-ray detector (Bioscan Flow-Count fitted with a NaI detector) coupled in series with the UV detector (Waters Model 996 set at λ 254 nm) was used for detection of radiolabeled products. Data acquisition for both the analytical and preparative systems was accomplished using a Waters Empower Chromatography System. MPC-6827 and desmethyl-MPC-6827 were synthesized according as previously reported [14 (link)]. [¹¹C]CO₂ was produced from a Siemens Eclipse cyclotron. Radiosynthesis of [¹¹C]MPC-6827 was achieved by reacting corresponding desmethyl-MPC-6827 phenolate with [¹¹C]CH₃I in a GE-FX2MeI/FX2M radiochemistry module using our established procedure [14 (link)–16 (link)]. All animal experimental procedures were carried out in accordance with the Institutional Animal Care Committee ethical guidelines of Columbia University Medical Center. All experiments were performed in male C57BL/6 J mice (Jackson Laboratories, Bar Harbour, ME) during adolescence and adulthood (postnatal day 35–120).