To evaluate the influence of TNF-α-induced MMP-9 expression on the metastatic activity of GES-1 cells, an in vitro Transwell assay (Becton-Dickinson, Franklin Lakes, NJ, United States) was used. Cells at a concentration of 1 × 105 cells/100 mL in serum-free RPMI 1640 medium were added to the upper chamber, and the Matrigel-uncoated insert was inserted in the lower chamber, which contained 20% FBS + RPMI 1640 medium. The migration assay was run for 24 h following a previously described standard protocol[39 (link),42 (link)].
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Evaluating TNF-α-induced MMP-9 and Metastasis
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Corresponding Organization :
Other organizations : Chang Gung University of Science and Technology, Chang Gung University
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Variable analysis
independent variables
- TNF-α-induced MMP-9 expression
- Metastatic activity of GES-1 cells
- Cell concentration (1 × 10^5 cells/100 mL)
- Serum-free RPMI 1640 medium in the upper chamber
- Matrigel-uncoated insert in the lower chamber
- 20% FBS + RPMI 1640 medium in the lower chamber
- Migration assay duration (24 h)
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