Molecular Characterization of DAAO Gene

DMEM, fetal bovine serum, antibiotic antimycotic solution, and trypsin-EDTA solution were purchased from Himedia (Thane West, Maharashtra, India). Hoechst 33342 and Trizol were purchased from Sigma-Aldrich (Burlington, MA, USA). Hydrogen peroxide was purchased from Merck (Darmstadt, Germany). RevertAid First Strand cDNA Synthesis Kit and TurboFect transfection Reagents were purchased from Thermo Fisher Scientific (Waltham, MA, USA). Restriction endonucleases were purchased from New England Biolabs (Ipswich, MA, USA). SYBR Green PCR Master Mix was purchased from Kapa Biosystems (Wilmington, MA, USA). Micro-amp Fast 96-well reaction 0.1 mL plates were purchased from Applied Biosystems (Waltham, MA, USA). QIAquick gel extraction kit was purchased from Qiagen (Hilden, Germany). Immobilon-P PVDF membranes were purchased from Merck-Millipore (Burlington, MA, USA). X-ray films, developer, and fixer were from Kodak (Rochester, NJ, USA). DAAO plasmid [26 (link)] was a kind gift from S.K. Goswami, School of Life Sciences, Jawaharlal Nehru University (New Delhi, India).