The ELISA was performed, as previously described [37 (link),38 (link)]. The segment of perihematoma in the ipsilateral cortex was obtained. The dissected tissues were homogenized in ice-cold Tris-HCl buffer (pH = 7.4, Solarbio, Beijing, China) and centrifuged at 13,000 Xg at 4°C for 10 min. ELISA was performed using commercial assay kits shown in Table 1 according to the instructions.
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