Quantifying Cell Apoptosis by Flow

Cell apoptosis was detected using the Annexin V-APC and 7-AAD Apoptosis Detection reagent (BD Biosciences) as previously described [21 (link)]. Briefly, 2 × 10⁵ cells per well were plated into 6-well plates and treated with 800 µM CoCl₂ for 48 h before treatment with nesfatin-1 at different concentrations (20, 60, and 80 nM) for another 24 h. Afterward, cells were washed and incubated Annexin V-conjugated APC and 7-AAD. Cell apoptosis was detected using a flow cytometer (Beckman, Pasadena, USA).

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Li M., Li K, & Ren Y. (2021). Nesfatin-1 protects H9c2 cardiomyocytes against cobalt chloride-induced hypoxic injury by modulating the MAPK and Notch1 signaling pathways. Journal of Biological Research, 28, 21.

Publication 2021

Annexin V-APC and 7-AAD Apoptosis Detection reagent

7 aad Annexin v Apoptosis Cell

Corresponding Organization :

Other organizations : Chongqing Jiulongpo People's Hospital, Xinjiang Medical University, First Affiliated Hospital of Xinjiang Medical University

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Variable analysis

independent variables

Nesfatin-1 concentration (20 nM, 60 nM, 80 nM)

dependent variables

Cell apoptosis

control variables

Cell density (2 × 10^5 cells per well)
CoCl2 concentration (800 µM)
CoCl2 treatment duration (48 h)
Nesfatin-1 treatment duration (24 h)

controls

Positive control: Not explicitly mentioned
Negative control: Not explicitly mentioned

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