Flow Cytometric Analysis of Immune Cell Populations

Flow-cytometric analysis of 100000 cells per sample was performed using a BD LSR II Flow Cytometer (BD Biosciences) following protocol as described in Dolgachev et al (2014)(56 (link)) and using the following antibodies Gr-1-PE, CD11c-APC-Cy7, F4/80-AF488, CD11b-PE-Cy7, and CD206-APC (BioLegend and BD Biosciences, San Jose, CA). Obtained data was plotted and analyzed using FlowJo software (Tree Star, Inc., Ashland, OR).

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Dolgachev V.A., Goldberg R., Suresh M.V., Thomas B., Talarico N., Hemmila M.R., Raghavendran K, & Machado-Aranda D. (2016). Electroporation-mediated Delivery of the FER Gene in the Resolution of Trauma-related Fatal Pneumonia. Gene therapy, 23(11), 785-796.

Publication 2016

BD LSR II Flow Cytometer Gr-1-PE CD11c-APC-Cy7 F4/80-AF488 CD11b-PE-Cy7 CD206-APC

Antibodies Cd11b Cells Flow cytometric Tree

Corresponding Organization :

Other organizations : University of Michigan–Ann Arbor

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Variable analysis

independent variables

Gr-1-PE
CD11c-APC-Cy7
F4/80-AF488
CD11b-PE-Cy7
CD206-APC

dependent variables

Cell population characteristics measured by flow cytometric analysis

control variables

Flow-cytometric analysis of 100000 cells per sample
Use of a BD LSR II Flow Cytometer (BD Biosciences)
Experimental protocol as described in Dolgachev et al. (2014)

controls

No positive or negative controls were explicitly mentioned in the provided information.

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