The immortalized BV-2 microglial cells (the Institute of Basic Medicine Chinese Academy of Medical Sciences, Beijing, China) were maintained in high glucose DMEM (Sigma, Saint Louis, MO, USA, Catalog No. D6429) supplemented with 10% FBS (Sigma, Saint Louis, MO, USA, Catalog No.12103C) at 37 °C in a humidified atmosphere of 5% CO2 in air.
Cells were seeded in 96 well plates at 1 × 105 cells per well, then cultured for 24 h. These cells’ group distribution and administration were divided into the following groups (n = 3 per group): control group; LPS (5 μg/mL) group; LPS + TAK-242 (1 μM) group (TAK-242, a TLR4 inhibitor which can prevent LPS-induce inflammatory response, was used as positive control [32 (link),33 (link)]); and LPS + dutasteride (10 nM–20 μM) group. Cells were incubated with TAK-242 (TargetMol, Washington, USA, Catalog No. TQ0181) and dutasteride (TargetMol, Washington, DC, USA, Catalog No. T1499) for 1 h, and subsequently stimulated with LPS (TargetMol, Washington, DC, USA, Catalog No. T11855) for 24 h, then stored for later tests.
Cells were seeded in 96 well plates at 1 × 105 cells per well, then cultured for 24 h. These cells’ group distribution and administration were divided into the following groups (n = 3 per group): control group; LPS (5 μg/mL) group; LPS + TAK-242 (1 μM) group (TAK-242, a TLR4 inhibitor which can prevent LPS-induce inflammatory response, was used as positive control [32 (link),33 (link)]); and LPS + dutasteride (10 nM–20 μM) group. Cells were incubated with TAK-242 (TargetMol, Washington, USA, Catalog No. TQ0181) and dutasteride (TargetMol, Washington, DC, USA, Catalog No. T1499) for 1 h, and subsequently stimulated with LPS (TargetMol, Washington, DC, USA, Catalog No. T11855) for 24 h, then stored for later tests.
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