Pulsed-field gel electrophoresis (PFGE) was performed as previously described [41 (link)]. In brief, The DNAs of PM1 and PM1-1 to PM1-5 were digested with SmaI (New England BioLabs, Ipswich, MA) and then were separated using a CHEF-DRIII apparatus (Bio-Rad Laboratories). PFGE was carried out at 200 V and 12°C for 20 h with the pulse times ranging from 5 to 60 s.
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