Murine Cytokine Quantification by ELISA

Murine cytokines were measured using a standard sandwich ELISA methods, as previously described [18] (link), [19] (link). The capture antibodies, detection antibodies and recombinant cytokines were purchased from R&D Systems. The ELISAs used in this study did not cross-react with other known murine cytokines. For lung cytokine measurements, lungs were homogenized in PBS containing 0.1% TritonX-100 and complete protease inhibitor (Roche) and centrifuged; the cleared supernatants were harvested and used for ELISAs.

Free full text: Click here

Xu Y., Ito T., Fushimi S., Takahashi S., Itakura J., Kimura R., Sato M., Mino M., Yoshimura A, & Matsukawa A. (2014). Spred-2 Deficiency Exacerbates Lipopolysaccharide-Induced Acute Lung Inflammation in Mice. PLoS ONE, 9(10), e108914.

Publication 2014

capture antibodies recombinant cytokines complete protease inhibitor

Antibodies Cytokine Elisas Lungs Murine Protease inhibitor

Corresponding Organization :

Other organizations : Okayama University, Keio University

Top 5 similar protocols

Variable analysis

independent variables

None explicitly mentioned

dependent variables

Murine cytokine levels

control variables

Capture antibodies, detection antibodies, and recombinant cytokines were purchased from R&D Systems
ELISAs used in this study did not cross-react with other known murine cytokines
Lungs were homogenized in PBS containing 0.1% TritonX-100 and complete protease inhibitor (Roche) and centrifuged; the cleared supernatants were harvested and used for ELISAs

controls

Positive control: None mentioned
Negative control: None mentioned

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!