Urine sediment culture protocol
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Corresponding Organization : University College London
Other organizations : Great Ormond Street Hospital, October 6 University
Variable analysis
- Sediment culture performed in parallel to enrich for microorganisms
- Microbial colonies identified by matrix-assisted laser desorption ionization time-of-flight mass spectrometry
- Centrifugation at 1400×g for 10 min
- Resuspension of sediment in 400 µL of sterile phosphate buffer saline (PBS)
- Tenfold serial dilutions performed in PBS
- 50 µL volumes plated onto ChromID CPS Elite agar
- Aerobic incubation at 37°C for 18–24 h
- No positive or negative controls explicitly mentioned
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