Sediment culture was performed in parallel to enrich for microorganisms, as this was previously shown to be more sensitive.25 (link) Briefly, 5–15 mL of urine was centrifuged at 1400×g for 10 min. The sediment was resuspended in 400 µL of sterile phosphate buffer saline (PBS) (Life Technologies, United Kingdom). Tenfold serial dilutions were performed in PBS, and 50 µL volumes were plated onto ChromID CPS Elite agar (bioMérieux, France). Cultures were incubated aerobically at 37°C for 18–24 h. Following incubation, microbial colonies were identified by matrix-assisted laser desorption ionization time-of-flight mass spectrometry (Bruker Daltonics, United States).
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Urine sediment culture protocol
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Corresponding Organization : University College London
Other organizations : Great Ormond Street Hospital, October 6 University
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Variable analysis
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- Sediment culture performed in parallel to enrich for microorganisms
- Microbial colonies identified by matrix-assisted laser desorption ionization time-of-flight mass spectrometry
- Centrifugation at 1400×g for 10 min
- Resuspension of sediment in 400 µL of sterile phosphate buffer saline (PBS)
- Tenfold serial dilutions performed in PBS
- 50 µL volumes plated onto ChromID CPS Elite agar
- Aerobic incubation at 37°C for 18–24 h
- No positive or negative controls explicitly mentioned
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