Contractile effects of Pg. Cr on rabbit jejunum

Rabbits were subjected to cervical dislocation. Their abdomens were opened. Their jejunums were removed and maintained in Petri dishes containing Tyrode’s solution, constantly aerated with Carbogen gas (95% oxygen/5% carbon dioxide). Portions of about 1.5 cm length of rabbits’ jejunal preparations were mounted in tissue organ baths. The tissues were stabilized in normal Tyrode’s solution for about 30 min. Following stabilization, Pg. Cr was tested on isolated rabbits’ jejunal preparations in concentrations 0.01, 0.03, 0.1, 0.3, 1.0, 3.0, 5.0 and 10.0 mg/ml [19 (link)–24 (link)]. Changes in isometric tension were recorded using force transducers (model MLT0201) coupled with bridge amplifiers FE221 connected to PowerLab 26/T (ADInstruments, Sydney, Australia). Data was recorded using Lab Chart 7 software (ADInstruments, Sydney, Australia). Intestinal responses were plotted as % of control.