The synthesis of hydrazides (BDTC1–BDTC7) was accomplished from hydrazinylthiazole esters via commercially available solvents and reagents. The solvents and reagents were purchased from internationally well-reputed chemical suppliers; glacial acetic acid, thiosemicarbazide, 3-nitrobenzaldehyde, 2-chlorobenzaldehyde, 4-chlorobenzaldehyde (Sigma Aldrich, USA), methanol, absolute ethanol, ethyl bromopyruvate, 3-chlorobenzaldehyde, 2-bromobenzaldehyde, 4-nitrobenzaldehyde (Merck, Germany), n-hexane, acetone, ethyl acetate (Riedel-de-Haen) and hydrazine monohydrate (Dae Jung, Korea).
The synthesis was initially determined by monitoring different physical parameters, like color change, melting point (MP), and retardation factor (Rf). Different spectro-analytical methods like Fourier-transform infrared (FT-IR) spectroscopy, high-resolution mass spectrometry (HRMS), and 1H- and 13C nuclear magnetic resonance (NMR) spectroscopies were used for structural confirmation. The purity and progress of the reaction product were monitored using TLC with silica gel 60 HF-254 pre-coated aluminum sheets (Merck, Germany). The approximate MP was determined with DMP-300 (A&E Lab, UK) apparatus. The absorptions in the IR spectra were used to determine functional groups and were recorded on a FT-IR spectrophotometer using attenuated total reflectance (ATR). 1H and 13C NMR data were recorded using Bruker Advance 300 MHz and Varian VNMRS 400 MHz spectrometers. A Bruker Micro TOF-ESI system was used to obtain the mass spectrometry data.
The synthesis was initially determined by monitoring different physical parameters, like color change, melting point (MP), and retardation factor (Rf). Different spectro-analytical methods like Fourier-transform infrared (FT-IR) spectroscopy, high-resolution mass spectrometry (HRMS), and 1H- and 13C nuclear magnetic resonance (NMR) spectroscopies were used for structural confirmation. The purity and progress of the reaction product were monitored using TLC with silica gel 60 HF-254 pre-coated aluminum sheets (Merck, Germany). The approximate MP was determined with DMP-300 (A&E Lab, UK) apparatus. The absorptions in the IR spectra were used to determine functional groups and were recorded on a FT-IR spectrophotometer using attenuated total reflectance (ATR). 1H and 13C NMR data were recorded using Bruker Advance 300 MHz and Varian VNMRS 400 MHz spectrometers. A Bruker Micro TOF-ESI system was used to obtain the mass spectrometry data.
