Visualizing c-MYC and Chromosome 8 in Tumors

The c-MYC gene was visualized by using a blue-staining system (ultraView silver in situ hybridization [SISH] dinitrophenol [DNP] detection kit and c-MYC DNP probe, Ventana Medical Systems, Tucson, AZ, USA). The centromere of chromosome 8 (CEP8) was visualized by using a red-staining system (ultraView red ISH digoxigenin [DIG] detection kit and chromosome 8 DIG probe, Ventana Medical Systems). Positive signals were visualized at 60 × magnification and counted in 50 non-overlapping tumor cell nuclei for each case (Fig 1) [17 (link)]. Small and large clusters were scored as 6 and 12 signals, respectively.

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Lee K.S., Kwak Y., Nam K.H., Kim D.W., Kang S.B., Choe G., Kim W.H, & Lee H.S. (2015). c-MYC Copy-Number Gain Is an Independent Prognostic Factor in Patients with Colorectal Cancer. PLoS ONE, 10(10), e0139727.

Publication 2015

ultraView silver in c-MYC DNP probe ultraView red ISH digoxigenin [DIG] detection kit chromosome 8 DIG probe

C myc C myc gene Cell nuclei Centromere Chromosome 8 Digoxigenin Dinitrophenol In situ hybridization Silver Tumor

Corresponding Organization : Inje University Haeundae Paik Hospital

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Variable analysis

independent variables

Staining system used to visualize c-MYC gene (blue-staining system)
Staining system used to visualize centromere of chromosome 8 (red-staining system)

dependent variables

Number of positive signals for c-MYC gene
Number of positive signals for centromere of chromosome 8

control variables

Magnification (60x)
Number of non-overlapping tumor cell nuclei counted (50)

controls

Positive control: Small and large clusters scored as 6 and 12 signals, respectively

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