Fluorescein diacetate (FDA) can be used to assess the cell viability of numerous cell types, as it generates observable differences in the fluorescence produced by live versus dead cells [22 (link)]. The collagen scaffold with implanted cells was incubated with 100 g/ml FDA for 1 min in the dark and then rinsed with phosphate-buffered saline. Samples were observed using the Zeiss 200 inverted fluorescent microscope (Carl Zeiss, Jena, Germany).
Cui Y., Yin Y., Zou Y., Zhao Y., Han J., Xu B., Chen B., Xiao Z., Song H., Shi Y., Xue W., Ma X, & Dai J. (2021). The Rotary Cell Culture System increases NTRK3 expression and promotes neuronal differentiation and migratory ability of neural stem cells cultured on collagen sponge. Stem Cell Research & Therapy, 12, 298.
Other organizations :
National Health and Family Planning Commission, Chinese Academy of Sciences, Institute of Genetics and Developmental Biology, Union Hospital, Jilin University, Annoroad Gene Technology (China)
Incubation of collagen scaffold with implanted cells in 100 μg/ml FDA for 1 min
dependent variables
Fluorescence produced by live versus dead cells
control variables
Rinsing the samples with phosphate-buffered saline after FDA incubation
Observing the samples using the Zeiss 200 inverted fluorescent microscope
controls
No positive or negative controls were explicitly mentioned in the provided information.
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