ChIP Assay for BCL6 and NAC1

ChIP extract preparation followed a previously described method with minor changes [35 (link)]. Briefly, target cells were seeded on 150 mm dishes and cultured until 90% confluence. Cells were fixed with 1% (w/v) paraformaldehyde prior to cross-linking with DTBP. Rabbit polyclonal antibodies raised against BCL6 (SC-858; Santa Cruz Biotechnology, Dallas, TX, USA) and NAC1 (SC-98638; Santa Cruz Biotechnology), or a rabbit IgG isotype (Abcam, Cambridge, MA, USA) were used for immunoprecipitations. The precipitated DNA was used for quantitative or semi-quantitative PCR. The primers used are summarized in Table 1.