In meso protein crystal growth

The crystals were grown using the in meso approach [62 ,63 (link),64 ], similarly to our previous work [41 (link),44 (link)]. The solubilized protein in the crystallization buffer was added to the mono-oleoyl-formed lipidic phase (Nu-Chek Prep, Elysian, MN, USA). Crystallization trials were set up using the NT8 robotic system (LCP version, Formulatrix, Bedford, MA, USA). The crystals were grown at 22 °C and reached the final size of ~100 µm within 1 week. The crystals were obtained using the precipitant solution consisting of 1.2 M KH₂PO₄/Na₂HPO₄ pH 4.6. Before harvesting, the crystals were incubated for 5 min in the respective precipitant solutions supplemented with 20% glycerol. All crystals were harvested using micromounts (MicroLoops HT, MiTeGen, Ithaca, NY, USA), then flash-cooled and stored in liquid nitrogen.