Comprehensive Characterization of Recurrent Glioma

Formalin-fixed, paraffin-embedded (FFPE) tumor tissue blocks were cut with a regular microtome for further immunohistochemical and methylation analysis. Immunohistochemistry for Ki67 was performed by an automated slide processing system (Autostainer Plus Link, Dako, Glostrup, Denmark; MIB-1/clone M7240, Dako) as previously described [8 (link)]. The Ki67 proliferation index was obtained by counting 500 cells in the most densely stained area and is given as a percentage (0–100%). Methylation analysis was performed of samples with resection at recurrence and differing histological features as compared to the first resection. DNA extracted from FFPE tissue was analyzed as described previously using Illumina Infinium HumanMethylation850 BeadChip (850 k) arrays (Illumina, San Diego, CA, USA) [9 (link)]. Samples were classified using the previously published MolecularNeuropathology classifier [10 (link)].

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Mair M.J., Wöhrer A., Furtner J., Simonovska A., Kiesel B., Oberndorfer S., Ungersböck K., Marosi C., Sahm F., Hainfellner J.A., Rössler K., Preusser M., Widhalm G, & Berghoff A.S. (2020). Clinical characteristics and prognostic factors of adult patients with pilocytic astrocytoma. Journal of Neuro-Oncology, 148(1), 187-198.

Publication 2020

Autostainer Plus Link MIB-1/clone M7240

Clone Embedded paraffin Formalin Immunohistochemistry Methylation Mib 1 Microtome Paraffin Recurrence Tissue Tumor

Corresponding Organization :

Other organizations : Medical University of Vienna, St. Pölten University of Applied Sciences, German Cancer Research Center, Heidelberg University

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Variable analysis

independent variables

Formalin-fixed, paraffin-embedded (FFPE) tumor tissue blocks

dependent variables

Immunohistochemistry for Ki67 (Ki67 proliferation index)
Methylation analysis of samples with resection at recurrence and differing histological features as compared to the first resection

control variables

Samples were classified using the previously published MolecularNeuropathology classifier

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