GnRHa-Induced Gonadal Responses in SYC

Samples (blood, pituitary, and gonad) were collected from both sexes of SYC, following in vivo administration of GnRHa (Sigma, St. Louis, MO, USA), as described previously [32 (link),47 (link),50 (link)], with a few modifications. Briefly, GnRHa was dissolved in distilled water and a working solution was prepared using 1× PBS. Both sexes of SYC at the ripen stage were divided into four groups. Three groups of SYC were injected intramuscularly with GnRHa, at concentrations of 10, 50, or 100 ng/100 g-bw (gram/bodyweight). In each treatment group, 15 SYC of each sex were injected. In the control group, 1× PBS was injected intramuscularly. Next, the pituitary samples from the male and female SYC were collected at 6 h, 12 h, and 24 h post-injection.