The antibody against TLR4 (#sc-293072) was from Santa Cruz Biotechnology (Santa Cruz, CA, USA). The antibodies against c-Jun NH2-terminal kinase (JNK) (#9252S), p-JNK (#9255S) and Caspase-8 (#9746S), anti-rabbit IgG HRP-linked antibody (#7074S), and anti-mouse IgG HRP-linked antibody (#7076S) were from Cell Signaling Technology (Danvers, MA, USA). The antibody against GSDMD (#ab219800) was from Abcam (Cambridge, UK). Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) antibody (#MB001) was from Bioworld Technology (Qixia District, Nanjing, China).
Western blotting was performed as we have previously described [64 (link)]. The proteins were transferred to polyvinylidene fluoride membranes (Millipore, Bedford, MA, USA), which were incubated with primary and secondary antibodies by standard techniques. The enhanced chemiluminescence (ChemiDoc XRS + System, Bio-Rad, Hercules, CA, USA) was used to accomplish immunodetection.
Western blotting was performed as we have previously described [64 (link)]. The proteins were transferred to polyvinylidene fluoride membranes (Millipore, Bedford, MA, USA), which were incubated with primary and secondary antibodies by standard techniques. The enhanced chemiluminescence (ChemiDoc XRS + System, Bio-Rad, Hercules, CA, USA) was used to accomplish immunodetection.
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