Immunohistochemical Characterization of HORMAD1 and STRA8

Immunohistochemistry was performed as previously described [9 (link),37 (link)]. Samples were incubated with HORMAD1 rabbit polyclonal antibody (Novus Biologicals, Centennial, CO, USA, NBP1-85401) or STRA8 (Abcam, ab49602; RRID:AB_945678) at a dilution of 1:500. Immunoreactivity was evaluated in Quantitative Pathology and Bioimage Analysis (Qupath; RRID:SCR_018257) using images of samples scanned on a Zeiss Axio scanner. Cell counts were performed randomly in three regions. The percentage of HORMAD1 or STRA8 immunostaining was used for analysis across 18 samples with corresponding positive and negative controls.

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Gantchev J., Messina-Pacheco J., Martínez Villarreal A., Ramchatesingh B., Lefrançois P., Xie P., Amar L., Xu H.H., Raveendra K., Sikorski D., Guerra Ordaz D.J., Gill R.P., Lambert M, & Litvinov I.V. (2023). Ectopically Expressed Meiosis-Specific Cancer Testis Antigen HORMAD1 Promotes Genomic Instability in Squamous Cell Carcinomas. Cells, 12(12), 1627.

Publication 2023

ab49602 Axio scanner

Antibody Biologicals Dilution Immunohistochemistry Novus Rabbit

Corresponding Organization : McGill University Health Centre

Other organizations : McGill University, Jewish General Hospital, Université Laval

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Variable analysis

independent variables

Incubation of samples with HORMAD1 rabbit polyclonal antibody
Incubation of samples with STRA8 antibody

dependent variables

Percentage of HORMAD1 or STRA8 immunostaining

control variables

Samples were scanned on a Zeiss Axio scanner
Cell counts were performed randomly in three regions

controls

Positive controls
Negative controls

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