Immunoblot Analysis of MAPK Activation

Immunoblot analysis of MAP kinase activation was performed with a polyclonal antibody to a synthetic peptide derived from the C-terminus of p44/42 MAPK (Erk1/2) (#9102, CST), Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (E10) Mouse mAb (#9106, CST), and IRdye-labelled secondary antibody and size-markers using the Odyssey Infrared (IR) imaging System (Li-Cor, Lincoln, NE, USA) according to the manufacturer’s protocol and as described by [68 (link),76 (link)].

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Zhou Y., Bennett T.M., Ruzycki P.A., Guo Z., Cao Y.Q., Shahidullah M., Delamere N.A, & Shiels A. (2024). A Cataract-Causing Mutation in the TRPM3 Cation Channel Disrupts Calcium Dynamics in the Lens. Cells, 13(3), 257.

Publication 2024

Odyssey Infrared (IR) imaging System

Corresponding Organization :

Other organizations : Washington University in St. Louis, University of Arizona

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Variable analysis

independent variables

Antibody used for immunoblot analysis (polyclonal antibody to a synthetic peptide derived from the C-terminus of p44/42 MAPK (Erk1/2) (#9102, CST), Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (E10) Mouse mAb (#9106, CST))

dependent variables

Activation of p44/42 MAPK (Erk1/2) as measured by immunoblot analysis

control variables

Use of IRdye-labelled secondary antibody and size-markers
Experiment performed using the Odyssey Infrared (IR) imaging System (Li-Cor, Lincoln, NE, USA) according to the manufacturer's protocol

positive controls

Not explicitly mentioned

negative controls

Not explicitly mentioned

Annotations

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