DU154 and LNCap cells were seeded into XF96 culture plates for ECAR and OCR analysis using the XF96 extracellular flux analyzer (Seahorse Bioscience). Glucose, oligomycin A, and 2-DG were automatically added for ECAR analysis. Oligomycin A, carbonyl cyanide 4-(trifluoromethoxy) phenylhydrazone (FCCP), and antimycin A and rotenone (Rote/AA) were automatically added for OCR analysis.
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