Antioxidant Effects of BA Nanoparticles

To determine the anti-oxidant effect of BA NPs on cells, NHA were randomly divided into 4 groups, which were treated with PBS, 2 μg/ml BA NPs, 10 μg/ml BA NPs and 30 μg/ml BA NPs. After 24 h, cells were lysed in RIPA lysis buffer containing protease for 30 min on ice. The protein concentration of each cell lysate sample was determined using the BCA and adjusted to equivalent amounts. Western blot analysis was performed according to the standard procedures as described in our previous report 29 (link), using antibodies targeting Nrf2 (Novus Biologicals), HO-1 (Novus Biologicals), and beta-actin (#643802, BioLegend). To determine the anti-oxidant effect in vivo, mice with successful MCAO surgery were randomly divided into 2 groups (n = 3 for each group), which received treatment of PBS or 2mg BA NPs, respectively. After 24 h, the brains were harvested, and the right hemispheres containing the ischemic area were excised. The brains from normal mice without surgery were used as controls. Western blot analysis was performed as described above.