Extracellular Vesicle Isolation and Quantification

EVs were isolated from culture supernatants using the ExoQuick-TC Ultra kit (SBI, Palo Alto, CA, USA) according to the manufacturer’s instructions. The protein content of the isolated EVs was determined using the Micro BCA assay kit (ThermoFisher Scientific, Oregon City, OR, USA), and their quantification was analyzed using the CD63, CD9, and CD81 ExoELISA-Ultra kits (SBI, Palo Alto, CA, USA), as recently described [27 (link),32 (link)]. For the treatment of cultured cells, 2 × 10⁸ EVs were employed.

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Nizar R., Cazacu S., Xiang C., Krasner M., Barbiro-Michaely E., Gerber D., Schwartz J., Fried I., Yuval S., Brodie A., Kazimirsky G., Amos N., Unger R., Brown S., Rogers L., Penning D.H, & Brodie C. (2023). Propofol Inhibits Glioma Stem Cell Growth and Migration and Their Interaction with Microglia via BDNF-AS and Extracellular Vesicles. Cells, 12(15), 1921.

Publication 2023

ExoQuick-TC Ultra kit Micro BCA assay kit

Assay Cultured cells Protein

Corresponding Organization : Bar-Ilan University

Other organizations : Shaare Zedek Medical Center, Henry Ford Health System

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Variable analysis

independent variables

Isolation of EVs using ExoQuick-TC Ultra kit

dependent variables

Protein content of isolated EVs
Quantification of EVs using CD63, CD9, and CD81 ExoELISA-Ultra kits

control variables

Not explicitly mentioned

controls

Positive control: Not specified
Negative control: Not specified

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