Renal Tissue MDA Quantification

The MDA concentration in renal tissues was measured according to a previously published protocol by our group (32 (link)). In short, homogenization and centrifugation of the renal tissues were performed at 8,832 x g for 10 min at 4˚C and the supernatant was collected and stored at -80˚C for MDA analysis. Subsequently, the MDA content of the renal tissues was measured according to the instructions of a commercial kit (TBARS assay kit; Cayman Chemical).

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Jawad A., Yoo Y.J., Cho J.H., Yoon J.C., Tian W., Islam M.S., Lee E.Y., Shin H.Y., Kim S.E., Kim K., Ahn D., Park B.Y., Kim I.S., Lee J.H, & Tae H.J. (2021). Therapeutic hypothermia effect on asphyxial cardiac arrest-induced renal ischemia/reperfusion injury via change of Nrf2/HO-1 levels. Experimental and Therapeutic Medicine, 22(3), 1031.

Publication 2021

TBARS assay kit

Assay Cayman Centrifugation Renal Tbars Tissues

Corresponding Organization :

Other organizations : Jeonbuk National University, Chonbuk National University Hospital

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Variable analysis

independent variables

Homogenization and centrifugation of the renal tissues at 8,832 x g for 10 min at 4˚C

dependent variables

MDA concentration in renal tissues

control variables

Temperature (4˚C) during homogenization and centrifugation

controls

Positive control: Not specified
Negative control: Not specified

Annotations

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