Genotyping SNP Markers using Sequenom MassARRAY

We genotyped the SNP markers (see Supplemental Table 1 for genotyped SNPs and information regarding their quality control) using Sequenom MassARRAY technology with iPLEX chemistry (Sequenom, San Diego, CA, USA) according to the manufacturer’s instructions. Primer information is available on request. We performed rigorous quality control as described earlier^{59 (link)}. We tested for pointwise genetic association by using a conventional 2 × 2 contingency table likelihood-ratio test⁶⁰ (LRT) of independence of SNP allele or genotype counts in cases and controls, as detailed earlier^{31 (link)}.

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Rouhiainen A., Kulesskaya N., Mennesson M., Misiewicz Z., Sipilä T., Sokolowska E., Trontti K., Urpa L., McEntegart W., Saarnio S., Hyytiä P, & Hovatta I. (2019). The bradykinin system in stress and anxiety in humans and mice. Scientific Reports, 9, 19437.

Publication 2019

MassARRAY technology iPLEX chemistry

Allele Genotype Iplex Primer Rigorous

Corresponding Organization :

Other organizations : University of Helsinki

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Variable analysis

independent variables

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SNP markers that passed quality control as described in Supplemental Table 1

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