We cultured fibroblasts derived from skin biopsies of three mitochondrial patients (P1, P2, and P3) harboring the following mutations:
We used control lines of primary human skin fibroblasts derived from healthy volunteer donors (C1, C2 and C3). These control cells were sex- and age-matched.
Patient and control cells were obtained following the Helsinki Declarations of 1964 (revised in 2001). Fibroblasts were cultured in Dulbecco’s modified Eagle’s medium (DMEM) (GibcoTM, Waltham, MA, USA) with 10% Fetal Bovine Serum (FBS) (GibcoTM, Waltham, MA, USA) and 1% penicillin/streptomycin (Sigma-Aldrich, Saint Louis, MO, USA) at 37 °C and 5% CO2. Cells were treated with polydatin and nicotinamide at 10 µM for seven days. All experiments were performed with cell cultures with a passage number lower than 10.
We used control lines of primary human skin fibroblasts derived from healthy volunteer donors (C1, C2 and C3). These control cells were sex- and age-matched.
Patient and control cells were obtained following the Helsinki Declarations of 1964 (revised in 2001). Fibroblasts were cultured in Dulbecco’s modified Eagle’s medium (DMEM) (GibcoTM, Waltham, MA, USA) with 10% Fetal Bovine Serum (FBS) (GibcoTM, Waltham, MA, USA) and 1% penicillin/streptomycin (Sigma-Aldrich, Saint Louis, MO, USA) at 37 °C and 5% CO2. Cells were treated with polydatin and nicotinamide at 10 µM for seven days. All experiments were performed with cell cultures with a passage number lower than 10.
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