Comprehensive Metabolite Extraction and Analysis

The solvent for extractions, LC-MS and NMR analysis, deuterium oxide (D₂O) plus TSP (4,4-dimethyl-4-silapentane-1-sulfonic acid, sodium dihydrogen phosphate (NaH₂PO₄), sodium hydrogen phosphate (Na₂HPO₄), sodium azide (NaN₃), potassium phosphate buffer, potassium persulfate (K₂S₂O₈), Folin–Ciocalteu phenol reagent, polyvinylpolypyrrolidone (PVPP), NaNO₂, AlCl₃, NaOH, rutin and gallic acid were purchased from Sigma Aldrich (Milano, Italy).

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Polcaro L.M., Samani M.R., Piacente S, & Masullo M. (2024). Development of a New Extraction Method for Pomegranate and Metabolite Profiling by a LC-MS and 1H NMR Combined Approach. Foods, 13(10), 1429.

Publication 2024

potassium persulfate (K2S2O8), Folin–Ciocalteu phenol reagent AlCl3 NaOH rutin gallic acid

Corresponding Organization : University of Palermo

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Variable analysis

independent variables

Deuterium oxide (D2O)
TSP (4,4-dimethyl-4-silapentane-1-sulfonic acid)
Sodium dihydrogen phosphate (NaH2PO4)
Sodium hydrogen phosphate (Na2HPO4)
Sodium azide (NaN3)
Potassium phosphate buffer
Potassium persulfate (K2S2O8)
Folin–Ciocalteu phenol reagent
Polyvinylpolypyrrolidone (PVPP)
Sodium nitrite (NaNO2)
Aluminum chloride (AlCl3)
Sodium hydroxide (NaOH)
Rutin
Gallic acid

dependent variables

Not explicitly mentioned

control variables

Not explicitly mentioned

controls

Positive control: Not specified
Negative control: Not specified

Annotations

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