Transcriptome Sequencing of Aplysia and Conus

We performed additional transcriptome sequencing of A. californica and Conus furvus. Specimens of A. californica were ordered from the National Resource for Aplysia at the University of Miami, FL, United States. Animals were anesthetized as previously described (Zhao et al., 2009 (link)). The venom gland of a single specimen of C. furvus was also dissected for sequencing. C. furvus was included in this study to provide an additional mollusk-hunting cone snail for the analyses. Total RNA was extracted using the Direct-zol RNA extraction kit (Zymo Research), with on-column DNase treatment and an additional wash step after the first purification, according to the manufacturer’s instructions. Library preparation and sequencing were performed by the University of Utah High Throughput Genomics Core Facility as previously described for different cone snail tissues (Koch et al., 2022 (link)). The SRA generated in this paper have been deposited with accession numbers SRR22829302, SRR23242094-SRR23242120.