The protein standards were all purchased from Sigma and included: α-casein (α-CN 23.6 kDa) from bovine milk (C6780-250MG, 70% pure), β-lactoglobulin (β-LG, 18.7 kDa) from bovine milk (L3908-250MG, 90% pure), albumin from bovine serum (BSA, 66.5 kDa, A7906-10G, 98% pure), and myoglobin from horse skeletal muscle (Myo, 16.9 kDa, M0630-250MG, 95–100% pure and salt-free) [28 (link),29 (link)].
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