The determination of s-CLL and l-CLL cells was conducted using FSC data and a back-gating strategy. The analysis was performed using a BD FACSCanto II (Becton Dickinson) instrument, and data acquisition was performed using BD FACSDiva software (v.8.0.2; Becton Dickinson). Flow cytometry data were analysed using FlowJo v.X0.7 software (Tree Star, Inc., San Carlos, CA, USA). In all the experiments, a minimum of 10,000 events was counted. The results were expressed as a percentage and mean fluorescence intensity (MFI).
Multiparametric Flow Cytometry of CLL Cells
The determination of s-CLL and l-CLL cells was conducted using FSC data and a back-gating strategy. The analysis was performed using a BD FACSCanto II (Becton Dickinson) instrument, and data acquisition was performed using BD FACSDiva software (v.8.0.2; Becton Dickinson). Flow cytometry data were analysed using FlowJo v.X0.7 software (Tree Star, Inc., San Carlos, CA, USA). In all the experiments, a minimum of 10,000 events was counted. The results were expressed as a percentage and mean fluorescence intensity (MFI).
Corresponding Organization : Palacký University Olomouc
Other organizations : National Academy of Sciences of Armenia, VSB - Technical University of Ostrava
Variable analysis
- CD183(CXCR3)-FITC
- CD20-PE
- CD5-PerCP-Cy5.5
- CD38-Pe-Cy7
- CD49d-APC
- CD19-APC-Cy7
- CD184 (CXCR4)-BV421
- HLA-DR-BV510
- Percentage of s-CLL and l-CLL cells
- Mean Fluorescence Intensity (MFI)
- Isotype-matched antibodies (BioLegend) as negative controls
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