MTS and BrdU Cell Growth Assays

For the cell growth test at different time points, the MTS assay was carried out with an MTS test kit (Thermo Fisher Scientific). A Wallac Victor 1420 Multilabel Counter was used to measure the absorbance. Each test was repeated 3 times. For the 5-bromo-2′-deoxyuridine (BrdU) test, cells were cultured for 3 days and then labeled with BrdU (3 μg/mL) (Thermo Fisher Scientific) for 4 h. The BrdU results were analyzed according to a previously described method (DeWaal et al., 2018 (link)).

Free full text: Click here

Lu Q., Ni Y., Wang W., Wang L., Jiang T, & Shang L. (2021). Dynamin 3 Inhibits the Proliferation of Non-small-Cell Lung Cancer Cells by Suppressing c-MET–GBR2–STAT3 Complex Formation. Frontiers in Cell and Developmental Biology, 9, 641403.

Publication 2021

BrdU

5 bromo 2 deoxyuridine Assay Bromo deoxyuridine Cells

Corresponding Organization : Air Force Medical University

Top 5 similar protocols

Variable analysis

independent variables

Time points for cell growth test

dependent variables

Cell growth/proliferation measured by MTS assay
Cell proliferation measured by BrdU labeling

control variables

MTS test kit (Thermo Fisher Scientific)
Wallac Victor 1420 Multilabel Counter for absorbance measurement
BrdU concentration (3 μg/mL)
Culture duration for BrdU test (3 days)

controls

Positive control: Not specified
Negative control: Not specified

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!