Immediately following the final expression test in each behavioral experiment,
subjects were briefly exposed to CO2 and decapitated by guillotine. Brains were
extracted and rapidly frozen in powdered dry ice. A series of 500-µm sections were
cut using an IEC Minotome cryostat, and NAc core and shell and caudate-putamen were
dissected using a combination of micropunch and microknife under an Olympus dissecting
microscope. Tissue lysates were prepared as described previously (e.g., Zheng et al. 2013 (link)) and stored in sample buffer in
aliquots at −80°C. The protein content was determined using the BCA
reagent kit with bovine serum albumin as a standard (Pierce, Rockford, IL).
subjects were briefly exposed to CO2 and decapitated by guillotine. Brains were
extracted and rapidly frozen in powdered dry ice. A series of 500-µm sections were
cut using an IEC Minotome cryostat, and NAc core and shell and caudate-putamen were
dissected using a combination of micropunch and microknife under an Olympus dissecting
microscope. Tissue lysates were prepared as described previously (e.g., Zheng et al. 2013 (link)) and stored in sample buffer in
aliquots at −80°C. The protein content was determined using the BCA
reagent kit with bovine serum albumin as a standard (Pierce, Rockford, IL).
