Leptin Signaling Pathway in Obesity

A detailed Methods section is available in the online supplement at http://circres.ahajournals.org.
Adenovirus expressing an enhanced green fluorescence protein (Ad-GFP), used as control, or both Ad-Cre and Ad-GFP (referred to as Ad-Cre) were microinjected bilaterally into the ARC of ObR^flox/flox mice. Leptin activation of Stat3 was analyzed by immunohistochemistry. Recording of SNA was obtained in anesthetized mice. Obesity was induced by high fat (45% kcal) feeding for 10 weeks.

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Harlan S.M., Morgan D.A., Agassandian K., Guo D.F., Cassell M.D., Sigmund C.D., Mark A.L, & Rahmouni K. (2011). Ablation of the Leptin Receptor in the Hypothalamic Arcuate Nucleus Abrogates Leptin-Induced Sympathetic Activation. Circulation research, 108(7), 808-812.

Publication 2011

Adenovirus Enhanced green fluorescence protein Immunohistochemistry Leptin Mice Obesity Stat3 Supplement

Corresponding Organization :

Other organizations : University of Iowa

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Variable analysis

independent variables

Adenovirus expressing an enhanced green fluorescence protein (Ad-GFP)
Both Ad-Cre and Ad-GFP (referred to as Ad-Cre)

dependent variables

Leptin activation of Stat3 (analyzed by immunohistochemistry)
Recording of SNA (obtained in anesthetized mice)

control variables

ObR^flox/flox mice
High fat (45% kcal) feeding for 10 weeks (to induce obesity)

controls

Ad-GFP (used as control)

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