Cell cycle was evaluated as previously reported [39 (link)]. Apoptosis assay was performed after 72 h treatment of emavusertib alone or in combination with ibrutinib by using eBioscience Annexin V Apoptosis Detection Kit (Thermo Fisher Scientific, Basel, Switzerland). Apoptosis induction and cell cycle distribution after the treatment were evaluated with flowCore R package from Bioconductor.
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