Chitosan Oligomers Production and Characterization

High-molecular-weight chitosan (CAS 9012-76-4) was supplied by Hangzhou Simit Chem. & Tech. Co. (Hangzhou, China). Neutrase^TM 0.8 L enzyme was acquired from Novozymes A/S (Bagsværd, Denmark). Chitosan oligomers with MW < 2000 Da were obtained following the procedure described by Santos-Moriano et al. [57 (link)], with the modifications reported in [58 (link)]. β-sitosterol (CAS 83-46-5, analytical standard), 3-hydroxy-2-methyl-4-pyrone (CAS 118-71-8), 5-(hydroxymethyl)furfural (CAS 67-47-0), methanol (CAS 67-56-1), tryptic soy agar (TSA, CAS 91079-40-2), and tryptic soy broth (TSB, CAS 8013-01-2) were supplied by Sigma–Aldrich Química (Madrid, Spain). Potato dextrose agar (PDA) was purchased from Becton Dickinson (Bergen County, NJ, USA).

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Sánchez-Hernández E., Buzón-Durán L., Cuchí-Oterino J.A., Martín-Gil J., Lorenzo-Vidal B, & Martín-Ramos P. (2022). Dwarf Pomegranate (Punica granatum L. var. nana): Source of 5-HMF and Bioactive Compounds with Applications in the Protection of Woody Crops. Plants, 11(4), 550.

Publication 2022

NeutraseTM 0.8 L enzyme Potato dextrose agar (PDA)

3 hydroxy 2 methyl 4 pyrone Agar Chitosan Dextrose Enzyme Furfural Methanol Potato Sitosterol Tryptic Tryptic soy broth

Corresponding Organization : Universidad de Zaragoza

Other organizations : Universidad de Valladolid, Hospital Universitario Río Hortega

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Variable analysis

independent variables

Chitosan oligomers with MW < 2000 Da

dependent variables

Not explicitly mentioned

control variables

High-molecular-weight chitosan (CAS 9012-76-4)
Neutrase™ 0.8 L enzyme
β-sitosterol (CAS 83-46-5, analytical standard)
3-hydroxy-2-methyl-4-pyrone (CAS 118-71-8)
5-(hydroxymethyl)furfural (CAS 67-47-0)
Methanol (CAS 67-56-1)
Tryptic soy agar (TSA, CAS 91079-40-2)
Tryptic soy broth (TSB, CAS 8013-01-2)
Potato dextrose agar (PDA)

controls

No positive or negative controls were explicitly mentioned in the provided information.

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