Serum concentration of sNfL was measured using an in house single molecule array (Simoa) method on an HD-1 analyzer (Quanterix, Lexington, MA), as previously described in detail by Gisslen [22 (link), 23 (link)]. Serum concentrations of tau and GFAp were measured using commercially available Simoa assays (Quanterix, Lexington, MA). All samples were measured as duplicates. The mean coefficient of variation of duplicate concentrations was 4.3%. In addition, a quality control sample was measured in duplicate on each of the seven runs used to complete the study. The intra-assay coefficient of variation for this sample was < 10%. All measurements were performed by board-certified laboratory technicians. The laboratorial analysis was conducted in a blinded fashion, as the assays were done without knowledge of the patient’s identity or diagnosis. Only one round of experiments was performed, using one batch of reagents.
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Serum Biomarkers Measurement Protocol
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Corresponding Organization :
Other organizations : Instituto do Câncer do Estado de São Paulo, Universidade de São Paulo, West Cancer Center, University of Tennessee Health Science Center, University of Gothenburg
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Variable analysis
independent variables
- Not explicitly mentioned
- Serum concentration of sNfL
- Serum concentrations of tau
- Serum concentrations of GFAp
- All samples were measured as duplicates
- A quality control sample was measured in duplicate on each of the seven runs used to complete the study
- The intra-assay coefficient of variation for the quality control sample was < 10%
- All measurements were performed by board-certified laboratory technicians
- The laboratorial analysis was conducted in a blinded fashion, as the assays were done without knowledge of the patient's identity or diagnosis
- Only one round of experiments was performed, using one batch of reagents
- Positive control: Quality control sample measured in duplicate on each run
- Negative control: Not explicitly mentioned
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