MiRNAs were extracted from cells by using the method described in our previous study 5 (link). Taqman probes (miR-93, U6) were acquired from the Taqman miRNA assay (Life Technologies, Carlsbad, CA, USA). Subsequent detection by qPCR was performed using the TaqMan PCR Master Mix (TAKARA, Dalian, China) with the StepOne Plus Real Time PCR Detection System (Life Technologies). MiR-93 expression was quantified by the relative quantification method with U6 as the reference gene and was verified by comparing with the control cells.
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Quantifying miRNA Expression by qPCR
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Corresponding Organization : The University of Texas MD Anderson Cancer Center
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Variable analysis
independent variables
- MiRNA extraction method
- MiR-93 expression
- U6 as reference gene
- Comparison with control cells
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